Tuesday, November 7, 2023

What does the GET / TRC pathway do? Crazy extensive proteomics to the rescue!


Have you ever really really really needed to get to the bottom of things? Like...you needed to see literally every tryptic peptide that an organism can produce that is bigger than 7 amino acids and smaller than 60? 

Looks like a group at Harvard was really tired of the GET pathway (called TRC) in humans and getting questions about it, so they knocked every protein in the pathway out in yeast and then pooled 96 (NINETY- SIX) offline fractions for MS3 TMT (aided by RTS) and got stellar quantification across an absurd number of peptides and proteins. 

I didn't even think yeast had 4,580 protein coding regions! Didn't someone work it out to just less than 4k just a few years ago? 

The data is ridiculously pretty, as you might expect from that much work on a relatively simple organism. 

Overkill? Maybe, but there aren't any question marks here on what each member of this pathway contributes to yeast biology. The TRC proteins in humans are involved in all sorts of different diseases, as well, so this can only be a solid resource for anyone interested in these proteins and what they do. 

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