Monday, May 17, 2010

58th ASMS in SLC

ASMS begins in SLC next week.  Unfortunately, due to the job switch there was no way for me to justify going this year.  Two of my coworkers will be attending and I can't wait to hear what happened there this year.

Saturday, May 1, 2010

Filter aided capture and elution (FACE)

This very understated paper in JPR may be the biggest advance in phosphoproteomics since the neutral loss scan.  This method outlines a new method, dubbed FACE, for Filter Aided Capture and Elution.
The process:
1) Digest all of your peptides
2) Incubate your peptides with an anti-phospho- antibody (they used 3G10 anti-phosphotyrosine)
3) Spin your peptides through a size-exclusion column.  The unbound peptides flow right through, leaving only the antibody and bound peptides
4) Wash to remove all unbound peptides
5) Elute your phosphotyrosine peptides using acid.

I see almost unlimited promise in this method.  I asked around and there are anti-phosphoserine and -threonine antibodies, but they are generally regarded as no good.
I will be trying this method very soon, considering phosphoproteomics is what I was hired for.