If EMT was on your "to do if I could talk a student into it next semester" list, I've got bad news for you!
This is a great model because it's largely cytoskeletal alterations as cells get a whole lot more resistant to just about everything. It's a lot of fun to do by things like IHC/ICC because you get these cool cytoskeletal proteins popping up you didn't see before.
This is also a cool study for the people who want to do SCP on a budget, because this is a nice application of the venderable Q Exactive Classic system. The study features a TIME COURSE! of TGFB treatment of these cells for 3 days and 9 days, so we'll be talking about this one in lab next week when we're back for that reason.
Time courses for SCP can be stressful. Everything has to go right for multiple time points? This isn't bulk proteomics time course where I can open up the incubator, pull out the media and dump in lysis buffer and wait for the next time point. You have to isolate those stupid cells while they're alive and then not lose your picograms of material before you go and run things. Batch effects, batch effects, batch effects.
Honestly, a beautiful study all around, even if I'm jealous I hadn't talked anyone into this one first.