Sunday, March 31, 2019
Hey! What's this thing? This is my INDUCTIVELY COUPLED PLASMA MASS SPEC thing that is being installed on Monday!
What does it do? It uses a frickin' argon plasma beam to convert anything (even metals!) to gases and then basically uses unit resolution to tell the elements apart.
It's pretty sensitive -- this white paper tells you how to set up an older model than mine (!!) to quantify how many molecules of cisplatin (a chemotherapeutic that has Platinum in it) are in each individual cell by turning each whole cell and into gas phase primary elemental components and measuring the Pt signal).
For those of you who don't live on continents that have banned the ElfSeverer journals -- there was just a special issue about it in one of their journals --
-- I can't access it either. I think the paywall just asked me for more money than Mario Kart for the Nintendo Switch -- and I don't yet have Mario Kart for the Switch.....yes...it's the same game as the Wi U...which I do have...but....
Sorry ElfSeverer....I'm going to read some other papers and -- whoa -- this is almost related - check this great new study out!
We use a lot of metal based drugs in the clinic. And we use them cause they kill cells we want dead. However, just like everything else out there, we often don't know the whole story.
Here, this team is interested in the mechanism of action of Plecstatin -- which isn't active until it's metabolized -- making the mechanism of action tougher to work out. New drugs based on ruthenium and osmium are kind of important.
As you might be aware -- Platinum is expensive. You can make a LOT more drug for the same price if you can replace Pt with another transition metal (Google says Pt is 30x more expensive than Ru) -- which is one reason to follow up on new drugs. According to this paper -- when they've done these switches it hasn't been exactly apples to apples. The drugs may be just as effective but the mechanisms may be a little different(!?!?)
They sample at multiple time points and use a load of techniques to figure out what is going on with this drug. All the ICP-MS is in the discussion and it sounds like it was used previously cause you can track the Ru easily with that technique. What they do here is pull-down shotgun analysis with a Q Exactive using biotin labeled Plecstatin-1 and activated drug.
The two drugs pull down very different populations of proteins -- with the prodrug interacting with proteins that just appear to be from initiating a generic stress response -- the activated form, however, is binding strongly to the target (Plectin?)
They do some further stuff where they modify the drug and repeat to try and figure out the important parts of the drug toward the mechanism of action they're interested in -- and how it changes over time and it appears to point to specific bonds as critical!
What is the moral of this story? Ben needs more hobbies? Probably. But maybe -- it's that ICP-MS can be a valuable tool for drug mechanism work and all sorts of other things (mine's primary job will be trying to protect young people from inhaling lead particles...a problem that is increasing rather than what it should be doing) but there is a bunch of other things it can do (and if you have some ideas where 3 minutes of intense ionization with an Argon beam might help you solve a puzzle? reach out! No joke -- it's 3 min per run. I'm worried about how to keep it busy!)
Saturday, March 30, 2019
How powerful is machine learning getting? For a distraction check out QuickDraw with Google. You draw stuff and a neural network thingy can even guess some of the stuff that I draw! (Those are mine above). It didn't get "beard" or "phone". It occurs to me that I forgot what a phone looks like.
For an example more relevant to the title of this blog -- check out this new preprint!
What did they do with machine learning? They learned biological stuff from bacteria and MALDI-TOF -- stuff about mechanism of action of different drugs on those bacteria!
Am I qualified to review this in any way? Nope. I flipped through the words, saw a lot of math and absorbed none of it. I can tell you they used a Bruker MicroFlex LT and only scanned from 2,000 to 15,000 m/z -- which seems like a smallish mass range for non-digested bacterial cells blasted with a laser -- but appears to provide sufficiently differentiating data. They also used some interesting programs in MatLab that I hadn't heard of for some of the pre-machine learning stuff.
In the end, I think it's a great Saturday afternoon read on something completely different than what I am supposed to be doing!
Friday, March 29, 2019
A pop sci article on this is available here.
The original study is available here.
Here in America this is of no concern to us. We're all trying to fit the biggest supercharger onto the least efficient V8 or V10 engine we can get dropped into the tallest truck we can, so we have 700 ft-lbs of torque (around 900 Nm to you fancy metric using literate types) with which to have flag hauling competitions.
However -- if you're one of those weirdos who thinks it would be cool if humans didn't just die in large numbers of things that we could prevent now by doing little things like using a little less fossil fuels (weirdos) -- maybe you should stop making fun of C and...wait...I just learned Pascal is still around...(efficient!!) and continue to make fun of thing like Perl and Java (NOT efficient!)
Or...re-engineer the basic architecture to get more efficiency, yo! If PASCAL can do it, I suspect the world can find a way to cut the energy usage of a Java Script in half. (Source: A guy who has compiled maybe 4 things in his whole life and is only awake right now thanks to generic DayQuil (a combination of strong stimulant, liver destroying pain killer and potent dissociative compound for sale over the counter in the US and labeled as a "cold medicine" -- for you fancy metric using literate types)
Wednesday, March 27, 2019
Throughout this blog you'll find whining about malaria proteomics. You may see it forever, until we finally beat this ancient disease. Any time I feel like I'm really getting good at this -- or the next generation of instrumentation is finally going to be the thing to blow this field wide open -- I end up deeply humbled by the experience. The people trudging through this full time will always have my respect.
And -- this new study from a lot of people I know -- and a name or two I hope to know soon -- just opened up an aspect of this frustrating organism that I would have thought impossible -- the MEROSOME Proteome!
Stolen image from this amazing text-book level Nature Review from 2008
The merosome is a stage between the Plasmodium leaving the liver (where they hide in low numbers) and work their way into the blood stream while somehow avoiding all immune responses. I don't think the mechanism is well understood at all, but the impression is that they hijack the membranes of liver cells and use those as their escape vessels.
To be clear -- this is a RIDICULOUSLY SMALL number of parasites. Ridiculously small.
How'd they get enough to work with? By doing an absurd amount of work. They grew one of the mouse model species of Plasmodium in cells in culture. By using a combination of microscopy and PCR -- they captured the merosomes and enriched them.
They normalize the samples by the genome amplification numbers. Ever had so little material that you had to AMPLIFY THE DNA to figure out how many cells were there? Me either -- but what a great idea. Honestly -- I'd guess if you had that little material -- even today's best MS technology wouldn't detect a thing -- and I'd be wrong.
This team uses SCX stage tips to fractionate the material -- and used single shot LC-MS (50cm EasySpray on a Lumos) using a method built for sensitivity (30,000 resolution MS/MS + 150ms max fill time -- not sure if max fill time override "use all available fill" was utilized).
Obviously there is going to be a lot going on here -- cell culture contaminants, human cell proteins, etc., so they included all those in a data analysis -- and scored almost 2,000 protein IDs(!!) from a phase of the malaria disease I wouldn't have believed possible to work with if I hadn't read this today.....
The downstream analysis might be equally impressive as the upstream work -- making this just a marvelous and inspiring study.
Sunday, March 24, 2019
I LOVE ABRF. I was a fan for years and I'm super psyched that I've gotten to participate in some way or another the last few years.
If you're a core director -- or want to be one -- and you aren't at least checking out ABRF resources you are doing yourself and you institution a disservice, yo! There are literally hundreds of people around the world who have the same job you do and ABRF is how they compare notes, both through the conference and through materials they put online and the projects that the work together (often completely remotely) on.
P.S. If you were at the last couple of ASMS things in San Antonio -- My gosh -- It's WAY nicer in March. 70F, rather than 120F.
And Sue Weintraub was here. And if you can't learn something from 10 seconds of talking to Sue that makes you, your research, and your core better -- maybe you should try another career. Your brain is toaster strudel -- time to go be a politician or administrator or something.
Lets be more positive -- ABRF 2019 SAN ANTONIO SPORADIC RECAP!
Someone honestly tried to trick me into believing talks started at 8am -- on a Sunday. Then at 9:30 when I walked over from my hotel with some other sciency looking people and --
--- 40,000 high school kids -- looking like they're applying for the circus or as stunt doubles for one of those Disney High School Musical things. (There were a LOT of injured dancers...) Did I fall for the greatest prank in history? Did these flow cytometrists as well? Turns out the convention center is just REALLY big and there is science on the other side. Favorite slide of the day (that wasn't mine)
iPRG 2019 is going to do MetaProteomics. HEY Protein Informatics People! They need help! Details will be posted here when available.
My talks were stupid, Nothing to learn there.
Brett Phinney did a talk and -- despite the fact he doesn't wear shoes -- he says REALLY smart things.
Do y'all know about BaseCamp? Brett said he couldn't imagine running a core without it -- it appears to be live updates for teams with a nominal cost. You know how you've got the one nocturnal weirdo in your lab who might fix stuff at 4am? He could just update BaseCamp that the weird ionization stability thing is fixed. So when the normal people come in at 4am or whatever the fuck they do -- they'd know that it was done! Smart!!
Brett had some software recommendations that I'm unfamiliar with, but excited to explore -- namely:
DIA-NN (the first N stands for NEURAL and the second one stands for NETWORK)
I learned that vDIA is something that I should be able to set up -- I'm just not smart enough. I'm exploring.
I got to see a talk by Birgit Schilling (which I might have misspelled) -- which is a highlight of any conference -- and hear about some recent work they're doing. Y'all should totally check this out!
How good is the wok in the Schilling lab? I'll stop making fun of SWATH for a day -- for real -- because if you're really really smart about it, you can honestly do science with it.
On that note -- what the heck is a SASP? And why do we need an Atlas? I don't know and neither does Google!
And the web address that Dr. Schilling put on her slides is DEFINITELY NSFW. DO NOT GO THERE. YIKES. I'll post a link if I find one.
Moving on -- have you seen EasyPep? I heard from sources I trust implicitly that we should all check it out. I heard it compared favorably to S-Trap -- and that is some serious praise. S-trap kicks ass. This requires verification.
Day 2: Do y'all know about Jove? New to me! Its a neat mechanism for publishing protocols with peer-reviewed VIDEOS! I'm excited about it.
Okay -- I have a load more to write -- but also this "job" thing to do. Maybe I'll write more later!
Saturday, March 23, 2019
Okay -- y'all know that depressing conversation, right?
You've prepped a ton of samples
You've phospho-enriched and fractionated and your QA samples are great and your internal QC standards are perfect.
You've crunched the data painstakingly to generate this amazingly well-curated list of proteins and their quan and the relative quan of thousands of phosphosites --- and you're presenting it to the people that procured these priceless samples and you hand over the results.
"Wait," they finally say, "Is that it?"
"You're not done, are you?"
"What am I going to do with a list of thousands of differential phosphosites?"
And you think, or if you're me, you actually say,
"No idea! Best of luck!" Remind them that they agreed to the hard stop so you could run to your next meeting!
Cause -- fuck if I know what to do next. Its been almost 10 years since I did my first SILAC phosphoproteomic study -- that OrbiXL only came back with a few hundred confident phosphopeptides. And a few dozen changed. So I looked 'em all up and it kindof made a story. You can't do it that way (well...I can't...I'm getting hella old) with 3,000 sites you got off of today's instruments....
WHAT IF THIS IS FINALLY THE THING THAT CAN MAKE SENSE OF PHOSPHO PATTERNS?!!??!
Check out this GitHub thing. It's full of amazing tools!!
Check out the MCP paper that describes them!
Then write me and tell me if this is finally THE THING WE'VE BEEN WAITING FOR! (Please) You know what I'm talking about. I'm not getting my hopes up. I've fell for it before. But -- for real -- maybe this is it!
Friday, March 22, 2019
Okay -- maybe I'm just special -- cause I logged into the TFS Cloud for the first time in a while and it had a popup that thanked me for being a valuable customer and told me my 10GB of Cloud Data Storage was now 1000 GB!! OR 1000000 MB
"More than millions --- thousands!!!" If you didn't see cold open of SNL last week -- 100% recommended.
This is also known as 1 TerrorByte (TB)
Proof. If you needed a reason to start integrating all your stuff and getting a hang of the TFS Cloud, maybe all this free storage is that reason!
And if it turns out I'm really just special? Sorry for getting your hopes up!
Thursday, March 21, 2019
New on the list of "things I'm super interested in, don't fully understand, wish I had time right this second to read in depth and -- HOLY COW I'm LATE FOR EVERYTHING but THIS IS WORTH POSTING -- list(?)"
ProtCID (which you can read about in this new PrePrint here)
You know alllllll that protein crystal stuff that people have been building up forever and exists in like 4,000 databases of varying quality and Googlability? You know allllllll that protein-protein interaction stuff that is pretty much the same? What if somebody was crazy enough to try and link it all together so you could access it in one place?
I think this is what this is!! Is that worth missing 1.28 meetings for? Siri says "ding-ding-ding-ding", which I'm taking as a confirmation, even if that's what she's been saying every 5 minutes for the last hour.
Tuesday, March 19, 2019
I was just made aware of the OSF recently -- when the best genome I've been able to find for a project we're working on was posted solely through this interesting/crazy mechanism.
You can check this out here.
I may get the details wrong but this is how I understand it.
1) Everything is posted on blockchain. You've heard of it, but probably wondered how the heck it might be useful? They use blockchain to host the drafts AND the reviewer comments.
2) The reviewer comments are openly available to everyone -- just as the results are
3) The reviewers appear to be paid for doing the reviews. "Paid" might mean imaginary internet points or cryptocurrencies that might have value today and not tomorrow. I don't know, but I feel like DASH has been around for quite a while now, though I've never investigated nor invested in that one myself. I learned my lesson with Garlicoin. ($1.14 I may never smell again...)
Monday, March 18, 2019
In case you were concerned, my obsession with metaproteomics is alive and well. For an update on the challenges and progress in the field that used PSMs to understand whole communities and ecosystems and global warming and who knows what else?!?!?
Check out this brand new review!
Is this everyone in this rapidly growing but still pretty small field? Hard to tell since the "rapidly growing part" and everything...but its more people than I knew were involved.
Sunday, March 17, 2019
Have you all been seeing DNA test kits everywhere? My local pharmacy has kits from 23andMe and much less expensive kits for all sorts of reasons that don't at all seem like crazy scams.
If you have been on the fence about sending your DNA off to be analyzed on the legal-in-exactly one country fully pirated next gen sequencers, I highly recommend you check out the DNA friend. Your results come back in minutes!
Friday, March 15, 2019
For first prize in the best chemical compound to say outloud today!
Nol (yeah?...yay?...nope. Neither work. On the 10th repeat I find myself saying "Nol-uh") I'll work on it on my commute.
What was I doing? Talking about a paper! This paper, in fact!
My good friend Dr. Blonder is a big fan of using organic solvents to get to membrane proteins (Scholar search "Blonder membranes" and you'll find papers doing things like digesting with trypsin directly in methanol and stuff going back to almost the 90s -- and these techniques do totally work) but a fluorinated solvent!??!?! That's at least new to me!
The interesting part is that this not only dissolves the membrane but it also fractionates the proteins, with integral membranes ending up separated in large degree from the anchor bound ones? That could be seriously useful when you're hunting seriously problematic proteins.
Wednesday, March 13, 2019
If this Tweet isn't something to be rampagingly optimistic about this morning -- I don't know what your problem is, but I hope you get better!
This is in reference to this article in some journal I've never heard of --
I'm way too behind to spend much time on it -- but they deconvolved a massive biological problem by using 110(!! a decent n?!?! for proteomics!?!?) pair matched samples (this is where you try your best to eliminate things that will muddy your results -- for example you compare normal to cancer tissue where the patient samples are the same gender and approximate age -- all things you need big sample sizes for) and with all this statistical power they realize they aren't just looking at one homogenous mixture of cancer patients -- there are different subtypes -- THEN when you break them into subtypes -- MARKERS start to become obvious!!!
Was that one very excited sentence? Probably!!
(I love that picture above, they look more excited about this study than me!)
Tuesday, March 12, 2019
Does the word "stoichiometry" give you awful flashbacks to adolescence? Have you avoided thinking about it by only using it in sentences like "phosphorylation only occurs at low stoichiometry" and not saying anything further? Time to fix that, because this is the topic in a biologically meaningful context from a brilliant and totally new (at least to me?) approach to understanding acetylation!
Are these sentences terrible? Daylight savings time is dumb and I feel even less coherent than usual.
How does acetylation in human cells happen? 2 ways
1) Tightly controlled enzymatic acetylation/deacetylation (acetyltransferase things?)
2) Non-enzymatic reactions on any available lysine from Acetyl-CoA just floating around.
This group described the abundance of #2 to be at a level where the super important tightly controlled cellular regulation focused #1 ends up being something really hard to define using our traditional method (they actually said "needle in a haystack" which is depressing)
And that's why they did a ton of innovative work to try and figure out which acetylations are which!
I'm not smart enough this morning (possibly ever, honestly) to explain what they did beyond the use of serial diluted SILAC (smart!) antibody enrichment of acetylation sites in conjunction with forcing chemical acetylation with 1M(!) acetyl-phosphate and doing loads of smart maths! (A QE HF was used for all the mass spec stuff and MaxQuant for data analysis)
All the data will be available (it isn't yet) at PRIDE here. (PXD009994)
What do we get? A massively better understanding of how and where and when acetylation occurs and which ones we REALLY need to pay attention to when trying to decide --> is this a downstream process caused by a normal (or glitchy) evolved mechanism OR is it just a highly abundant protein that looks important because of chemical acetylation effects?
Sunday, March 10, 2019
The reason to be excited about this great new paper In Press at MCP is the subtitle on the top of each page:
The biologists have been super excited about these things for a long time -- and probably more than a little disappointed in what proteomics has done to help them understand this ultra important class of PTMs.
Does this fix it? I don't know how many sites people will want/need or how many exist, but this sure looks like a ton of data.
For us mass spec nerds, maybe the most interesting part is the surprisingly complementary results generated by ETD and EThcD. In my head I kind of consider the two about the same thing. ETD makes mostly charge reduced species, but if you look close enough you'll find c and z ions. EThcD makes less charge reduced stuff, that -- in theory -- is replaced with a blend of c,z,b and y fragments -- but my experience is that Sequest can't make any sense out of it.
All this data processing was done in MaxQuant and allowed a dizzying number of dynamic mods:
This results in some beautiful data -- tons of sites identified and localized and data that ought to make the biologists a whole lot happier than anything I've seen before!
Saturday, March 9, 2019
Okay! This is more of what proteomics needs! More sophisticated and better chromatography needs to sneak in here and there and this great note is a perfect example of why.
My heart sank when I realized a lot of people were breaking out the SCX columns to enrich for their crosslinked peptide species. I'm sure SCX still has uses out there in the world, but when I hear those letters all I think of is putting in milligrams of peptides and getting micrograms back, so maybe this is the answer?
Friday, March 8, 2019
How much better are laser pointers now than they were like 10 years ago? I can get a laser at the dollar tree (if you don't have these -- they're amazing. Every item in the store costs $1 and the items stocked are chosen completely at random. Seriously. Go into one and try to cap yourself at $7. I bet you can't do it) and 1) it's way brighter than the ones even a few years ago 2) the batteries last longer and 3) it's $1. Which is crazy.
What about a real use of lasers, like laser microdissection? Could it possibly be improving at the same rate? Or -- at the rate that proteomics technology is improving?
What if you used the best of both? And you painstakingly optimized EVERYTHING necessary to link the two techniques together? Then you'd have this new paper in JPR.
I'm assuming if you have a Lumos system and you're using 50cm columns on it, you didn't get your laser at a Dollar Tree. I'm also assuming that if you work your way upwards toward identification of 1,500 human proteins(!!!) from stuff you cut with a laser from a slide of tissue that is 10 micrometers thick (probably 1 cell width, right? Google is confused by the question) that I'm not the only person who is super impressed.
The ion trap in the Lumos was used for a lot of the MS/MS (the sensitivity comes in handy when you're trying to resolve individual cell types off slides) and MaxQuant/Perseus/fancy R stuff was used to pull the story all together.
I'm unclear regarding the isolation of specific cell types and what they are, but in one set of samples this group came close to 4,000 proteins ID'ed!!
If it's been a few years since you last used laser microdissection + proteomics and you know someone with a question that only these techniques could answer, maybe it's time to get a new slide and follow the protocols in this new paper to the letter!
Thursday, March 7, 2019
I need to add some stuff to my bucket list so I've got more reasons to not die than the fact there is probably an elderly dog out there in the world with diabetes, allopecia and incontinence that needs me.
2019 is rocking here in Columbia, MD!! ---
Invited talk(s) at ABRF!! ("Making a core lab nimble and efficient" and "introducing the WIN antibody characterization community project" -- actually, this needs it's own sentence...)
Here is the first slide!
Are you guys getting inundated with antibody and antibody drug characterization requests?!? If you aren't, there are probably people at your university or facility who are doing this and sending it elsewhere.
In the U.S. like 2 months ago -- FDA cleared MoxywoxytootymomoloopyMab and it's an antibody with powerful drugs on it -- it localizes to CD22 (I think) on cancer cells and then blows them up. BOOM. And there are dozens like it trying to get clearance. Antibody drugs are coming down the pipeline like crazy AND mass spectrometry is the only way you can make sure 1) verify the sequence identity 2) verify that drug conjugations were successful
Here is the idea for the community study -- How is everyone doing this?!??! I started counting them up and I came up with over a dozen different combinations of ways that you could conceivably characterize a monoclonal antibody with mass spectrometry. What if we (ABRF WIN!) found labs that do this -- send them antibodies -- and we all work together to figure out: 1) How everyone is doing it 2) What is the best way?
If you were on the fence about being in San Antonio for ABRF (I know...what are the odds the Spurs have 3 games on the road....?) maybe the fact that you've got 100 requests to do mAB characterization in your inbox you're ignoring is a reason to go?
AND I JUST FOUND OUT TODAY --- I'M SPEAKING AT ASMS!!! I speak on Thursday! YES, I also thought the conference ended on Wednesday. It doesn't! I have to change my AirBnB and flights! I think one organizer was like -- "come on, just let Ben have a milk crate to stand on outside the convention center on Friday or something. He's been making requests to talk for like 18 years. Who knows, he might be too old to travel next year...." THANK YOU ORGANIZERS!!
I'm speaking on the work Conor Jenkins and I are doing with OptysTech using stupid amounts of their cloud processing power to find cancer mutations -- without any genetics based sequencing required. There is a preprint with a few details out now and some super promising results in hand that we're working on clarifying/verifying.
THANK YOU ORGANIZERS! I LOVE TALKING ABOUT MASS SPECTROMETRY!!!
Tuesday, March 5, 2019
Is the super fat squirrel thing happening everywhere? In my back yard they're so round now that even my not-so-athletic dogs seem like they might actually catch one. It occurred to me that it's weird to see squirrels in the middle of the winter. Don't they hibernate or something? According to a lot of articles I found online (IFLS link) it is actually happening everywhere.
Okay -- so that was my question -- what about a real science question that I never would have thought to ask? How does the squirrel metabolome change during hibernation? For the answer you'll need to check out this new paper at JPR.
It turns out that Arctic squirrels (which, I'm pretty sure was also a band that my housemate in grad school was a fan of) do definitely hibernate -- and make a good model for studying hibernation.
I assume it's easier to get blood from an arctic squirrel than from one of these things....
(Wikipedia informs me that a bear isn't a true hibernator, but the joke still holds, IMHO)
For the sciency parts --- blood was drawn from the squirrels both in full torpor (what I'd consider hiberation ) and coming out of it and then also fully awake and other time points and this is the samples that were used for metabolomics.
This team focused on the metabolic shifts in the red blood cells! I don't read as many metabolomics papers, but this isn't something I've seen before. The RBCs were separated and lysed and this is what went on to the UHPLC Q Exactive system.
When I do read metabolomics papers, I'm always surprised by how low the TopN is. You often see the Top3 or Top5 most abundant ions selected for fragmentation. The now possibly discontinued(?) (and awesome) Q Exactive Focus system was only capable of doing Top3 when it first launched. Unless you're doing UHPLC with crazy sharp peaks, this always seems like a waste of cycle time to me. This group uses a 70,000 resolution followed by a Top15 method for MS/MS acquisition. Rough math in my head says a 1.3 second cycle time if 15 ions are actually selected (which won't be often) and I can't see a downside to this (forget FWHM for peaks for high res MS1, you want baseline to baseline and this is going to still provide plenty of MS1 for accurate quan).
For the downstream processing, Compound Discoverer was used with a ton of databases (yay! if you have them use them!) including NIST, KEGG, LipidMaps, and an in house library of 1,000 compounds. MetaboAnalyst (something I need to check out and keep forgetting) was also employed here.
What did they get from all this work? A really interesting and surprisingly complete picture of the RBC metabolism in and out of hibernation, including some targets that they were able to get standards for and verify with absolute quan techniques.
Did I decide to read this paper initially because of the fat squirrels in my back yard? Yes.
Did I learn a lot about how to put together a solid metabolomics study as a consequence? Also yes!
Added bonus: I discovered that there subreddit that is completely devoted to insulting fat squirrels (of course there is...?). The goal seems to be to insult fat squirrels with the most profanities you possibly can, to the point that you have to be 18 or older to enter the site, and I probably shouldn't direct link to that.