Tuesday, November 8, 2022

Optimized diaPASEF for phosphoproteomics!


This is great! Does anyone have one of these for ADP-ribosylations? I have yet to see one in our data, and we can't tell if the TIMS is too rough on the mod (in-source decay?) or if they move funny in ion mobility space. 

This group claims 35,000 phosphorylation sites in 44 minutes! Out of DIA data

They did this by creating project specific phosphoproteomics data with DDA processed through MSFragger (!!!) and then they used this program (pyDIAid) to create their method (!!!)

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