Doing mitochondial proteomics? You're going to be excited about this new resource!
How 'bout:
Overview of instrument methods from different vendor nanoLC and MS systems?
Step by step data analysis methodologies and rationale?
What about mitochondrial proteome reference datasets you can get from ProteomeXchange (here)?
That do anything for you?
How 'bout the power to kill a yak from 200 yards away with...mind bullets? Sorry...Reddit reminded me of this video and the song has been stuck in my head all week.
The construction of the mitochondrial reference pathways and data processing schemes alone make this a super valuable resource. The fact that you can check your instrument setup and essentially follow the established protocols and know what to expect from your data because of the RAW files they deposit -- that's skadoosh.
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