Need some files to compare between different instruments or to test your pipelines? This recent preprint probably has you covered. 700 instrument files with tons of replicates and QCs! DDA vs DIA vs fancy things are are DIA with little tweaks and funny names? It's all here!
Huge shoutout to Dr. Neely for tipping me off to this new study and tool!
I send anyone who asks for this stuff to.....oh...fuuu... Okay, well, I used to have a place to refer anyone who asked but I think there is just a beautiful empty lab there since everyone there was recruited to industry.... Well, maybe I will find out soon!
Hey you! Do you think you need that new ultra-expensive fancy pants instrument to compete with today's flashiest hardware? It might seem like an unending rat race in a field largely driven by instrument vendors who somehow maintain possession of the ever-dwindling supply of real mass spectrometrists (the ones who have built or significantly altered instrument hardware before, which is a distinction in some branches of mass spec, where the rest of us are "users").
Or. Have we still not tapped the full potential of instruments that are rapidly approaching a decade out in our hands?
I have never once in my life gotten anywhere near 8,000 proteins in an hour and I've got access to some flashy new stuff these days.
All the files are open on MASSIVE here!
Hey you! Are you bored at HUPO? Are you not attending HUPO, but want to see tons of ASMS posters?
Today and tomorrow anyone with an ASMS login can go and see all the posters! You can check that out here.
Okay, so here is an initiative that we should all be excited about!
This extremely well-written and concise review is something we should be sending around (if your facility isn't already operating at 200% capacity thanks to all this new excitement about proteomics!) to justify those top-down focused instruments!
This might largely be for me, but there are some metaproteomics people out there! So many that they just had a conference, I think!
Ever just ended up with a big batch of de novo results or matching your proteomics data to some RNASeq /PacBio or NanoPore data and instead of getting an accession that is useful you just have 00034634543563459054P0000P203940938430294 and thought about pBLASTing every one individually to figure out what the heck it does?
DON'T DO THAT! (I mean, do what you want, but after the 10th one I bet you'll be rethinking the missteps you've made in your career that led you to that moment). Then Egg-NOG it! And there are some amazing new updates for it.
After dumping a relatively large amount of time and personal funds into SARS-CoV-2 peptide detection early in 2020 (and subverting some great people to help me!) I ended up with some COVID research fatigue. I know people are still doing great work out there, and here is a nice recent example!
If you also have some fatigue but also curiosity about targeting the virus, this group goes back to the beginning and critically reviews what work, didn't work and why and fills in a lot of gaps for any future work toward viral detection.
I've got almost as many unfinished blog posts as manuscripts open on my desktop and some pressure to wrap up some things before others, but -- holy cow -- this is fantastic!
Not only do they pull off sequencing a protein, but pull it back in reverse it to do it again! Full de novo single protein sequencing from a single protein molecule. Sure, it's slow, but have you seen their solutions for speeding up their DNA sequencers?
MP321 -- Using a TMT method called Tomahto to go after "Dark kinases" (did you know we still don't even know all the kinases?? WTF?
MP312 -- A really cost effective looking way to multiplex up to 32 samples
MP331 -- Depleted and TMT10-plex labeled high pH offline fractionated plasma from diseased children. One of the deepest plasma proteomes I've ever seen. Pretty downstream analysis
MP345 -- Whoa. TMTc looks really stellar compared to even MS3 based quantification? I need to hit this group up for some help with their Github stuff.
MP348 -- Someone actually did the video upload! Understudied organism (lake trout) brain proteomics? in the context of evolution and polution accumulation? Impressive work and from an undergraduate researcher!
MP333 -- A new TMT QC standard! I love the TKO standard! This looks to me like it's the TKO standard taken to a whole new level with quantification involved as well! Wait. WTF is Proteome Discoverer 3.0?
MP051 -- An absurdly pretty histone marker analysis!
MP041 -- Luxon (the laser electrospray thing) analysis of serotonin in plasma
MP017 -- A great argument for serum glycomics!
