This is a nanoLC volume calculator inspired by the AMAZING (but dead?) MSBioWorks phone application I miss a lot and made with the frustrating assistance of some AI thing my university lets me use. It's honestly pretty good at color palettes, even if it's bad at math and confident/arrogant about it.
nanoLC System Volume
To use this, I've assumed some things about your flow path. You'll need to select how many columns and tubing sections. Let me know if I'm not considering modern nanoLC options, yo. It'll autofill some normalish stuff, so you'll need to fill in the inner diameter and length for each. It sums everything into total system volume live.
00
Set Up Your Flow Path
Sections 02 and 03 below build themselves to match — enter the inner diameter and length for each capillary as it appears.
Line weight and column shading scale with each segment's volume — thicker lines and denser fill hold more mobile phase.You'll have to believe me because I can't figure out why these fonts are OUT OF CONTROL
Total system volume
0.0 nL
01
Injection Loop
0.0 nL
02
Tubing Sections
0.0 nL total
03
Columns
0.0 nL total
04
Additional Dead Volume
0.0 nL total
—
Breakdown
Internal Dead Volume
Injection loop + pre-valve tubing
0.0 nL
0.0% of total
Operational Dead Volume (Probably the one you care about)
Valve → columns → MS inlet
0.0 nL
0.0% of total
| Component | Zone | Volume | Share | % |
|---|
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