Is that a cell surface protein? Oh yeah, sure it is, someone in 2003 BLAST'ed the genome sequence for it and found a yeast cell surface protein with 12% sequence homology....
And....that may honestly summarize some percentage of "cell surface proteins" we have on our lists. Legitimately somewhat informed guesswork based on organisms separated in evolution over 1.5 billion years. Or something.
Of course people have built off of these guesses by raising antibodies and verifying by microscopy, etc., but we have never had an easy way to rapidly annotate mammalian proteins as truly being on the cell surface.
UNTIL NOW. Introducing Veneer!
There is a lot to unpack in this great new resource. The background of the resource was constructred from data from over 4,000 separate publications!
"There are many different ways to enrich cell surface proteomes, I bet this only thinks about the one this group uses"
Shown here are the Veneer processed results from 4 separate enrichment methods!
It doesn't take much imagination to think what Veneer could do for the biology community, right? I've never made an antibody before but my impression of it is that it is 1) expensive 2) takes forever and 3) requires you to go all vampire on 700 bunny rabbits, 11 camels and possibly 1.5 cheetahs. If you need to make a cell surface antibody and that protein really isn't a cell surface protein OR you are raising the antibody to a a section of that protein that is not actually the part ON THE CELL SURFACE. That's a lot of bunny / camel /cheetah blood for ....absolutely.....nothing......
Vaneer doesn't have to be perfect to have a major effect on efficiency. Anything would help drastically but they obviously put loads of thought into this amazing new resource. If you're swamped for time, you don't even have to read this paper. Check out the logic summarized in Figures 2 and 4.
Or just download Veneer from this Github and run it (might be a minor UTF-8 issue for Macs) now!
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