Spectral quality overrides peptide scores!

 

I've got some great new people who are new to proteomics around and I get to forward and think about this fantastic tutorial again.

I don't know when scientists got so obsessed with tiny numbers far to the right of decimal places, I think the BLAST score is probably to blame in biology. This tutorial is fantastic because it reminds you that the mass spectrometer doesn't actually generate p/q/r -values, it generates m/z ratios and intensity values (I mean...that's what you see on the PC attached to it most of the time..). It might be more fantastic because of how approachable it is.