Ion-mobility fractionation?

 

Different types of gas phase fractionation methods pop up here every couple of years, but this is the first one I've seen that breaks up the ion density using TIMS.

The results really illustrate how dense the peptide signal is. Remember when we were trying to get to a point where we could fragment 100,000 peptides and that seemed way off in the future? 

These authors use extremely narrorow ion mobility ranges for multiple injections on a TIMSTOF Pro and crank their protein IDs way up. To demonstrate further utility they use the sum of these runs to generate a spectral library for pasefDIA and improve those results relative to other methods of library generation. Clever idea with solid results worth thinking about.