Wednesday, July 6, 2022

A true multi-omic analysis of CBD on cells in culture!


Well...this wasn't what I expected to run into on MCP this morning.....

"Multi-omics" gets thrown around a lot. People with sequencers often mean "we did genomics & transcriptomics" and if you're lucky they threw in a DNA methylation array or something.

This group did MULTI-OMICS.  

Global and subcellular fraction proteomics was performed using TMT11-plex using an Orbitrap Fusion with what appears to be a somewhat complex way of describing the MS3 workflow. They had to pack them into 3 separate plexes and mixed up the channels to help with missing values between plexes. 

I haven't done SILAC in a long time because it is so much freaking work, but I ain't about to fault anyone for doing their phosphoproteomics that way. It was called the gold standard for a long time for very good reasons. 

The metabolomics and lipidomics were separately performed using a Q Exactive with UHPLC. I'm a little surprised that those original files don't appear to be on a repository because there is over 1 TERABYTE of data on massive to support the proteomics. The transcriptomics is also up on GEO. I was mostly curious to see the original chromatograms because I haven't used the columns they employed here.

What they come up with after all this work is an impressively defined story for what this compound does to cells in culture. Now....always worth considering that a lot of things do really powerful things to cells in culture. Resveratrol massively boosts the lifespan and health of cells in culture, but delivery has never been sufficient (that I'm aware of) to have any positive effects in humans. CBD does appear to have some really interesting phenotypes here, but it is worth keeping in mind that it won't come off a C-18 column with less than 80% methanol, so delivery mechanisms are a concern for extrapolating medical effects.

This is an impressive study all-around and a great example of real multi-omics. 

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