This is a really interesting study new study on a lot of levels. Now, it is required under the rules of this blog that I insert at least the following gif.
There is a ton of work here, but why would you want to read it? Well, it does a really good job of integrating a ton of iTraQ 8-plex data from a Q Exactive Plus. Offline fractionation is involved as well as a ton of different time points and conditions.
I found this just a really enjoyable work with a complicated series of goals that succeeded thanks to a rigorous experimental design, that pays off in the end with some cool biology. Highly recommended!