Friday, July 9, 2021

Rapid analysis of small amounts of heart tissue with Azo and PASEF!


I've got to move fast and can't do this great new study justice. Have you ever tried heart proteomics? It isn't a ton of fun. There are just a couple of proteins that make up just about the entire proteome. Unless there have been new developments, there aren't easy depletion kits. Most high coverage proteomics is long 2D experiments starting with tons of material -- or -- just 4 million Titin peptides. 

But if you want to understand what is going on in the heart, you have to go to the protein level. A lot of the cells rarely divide so you aren't exactly dealing with a lot of genomic instability issues like in cancer, and that's why this new study is so cool!

1) Low amounts of sample (they work down to 1 milligram of heart tissue. Not protein. Starting material!) 

2) It's fast! They optimize a sample prep method that gets the digestion conditions both reproducible and down to 30 minutes? What?

3) It's 1 dimensional! (or 4, depending on how you count, I guess) Two hour gradients on a TIMSTOF Pro. 

And it gets some serious depth of coverage. 4,000 proteins? 

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