Okay, so even if this is possible after I've repeatedly said it probably isn't (yet?), I hope we can all look at this and agree that it looks like a complete pain in the butt to actually do. But...I don't like bulk phosphopeptide enrichment, so I have my biases.
Now, you could probably argue immediately that 83 phosphopeptides from one HeLa cell isn't very much. And I'd also agree with that, but it's probably more than I could get without enrichment. Though....I might legitimately see if that is true on an Ultra2. We do have some cancer cells coming in....
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