Oh. This is really cool. I'm so glad that SCIEX is finally getting some traction with their super cool ZenoTOF hardware. The high speed high resolution mass spec world is really super competitive right now. You really can't make a bad choice (aside from Agilent, obviously - hey, I didn't tell them to abandon global proteomics instrument market -they're doing fine in their chosen niches) for getting amazing proteomics data.
There is exactly one instrument out there that has big ass magnets inside it that forces charges onto your peptides for democratic fragmentation. I was supposed to evaluate a ZenoTOF for single cell proteomics, and it was good enough to get a paper in just a couple of months with it but the super sensitive fast PRMs (which got us a super cool paper in the same time period) and EAD were what really wow'ed us.
Could you do even more with EAD, though? What if while you had those ions you also applied another collision energy? Could you really bust those molecules up to get complete coverage of the peskiest ones? Importantly, could it still be way faster than other democratic fragmentation methods (that use chemical based fragmentation)?
Pretty much! Best I can tell they optimize these glycopeptides out and they do need to slow it down some. It looks like the best data is coming off between 13 Hz and 19 Hz (my math from the method section details). They have some time for the EAD and some time for the CID and some accumulation time and that sums up.
I don't know what the new Exedrin ETD benchtop instruments are getting with their improved (and seemingly incredible) new Orbitrap hardware. Given I'm used to 100ms reaction times for ETD not counting the Orbitrap scan times, and internal HCD cells (IRMs?) stop, gate, go times. I think this has to still wildly competitive.
Probably also worth considering that the 7600 this was tested on is now 2 generations behind the faster and more sensitive ones. So...I suspect you could go even faster on the new ones?
No comments:
Post a Comment