Are you ready for deep visual lipidomics? No? Okay what about normal spatial lipidomics with deep visual proteomics?
The diagram pretty much shows what the paper did, but the co-registration of the spatial data from the laser capture microdissection work (now called deep visual proteomics, y'all, get on the hip terminology) to the lipidomics is a star. This does come from the very small but valuable bank of post-mortem human brain tissue in Baltimore that one of these authors definitely didn't steal from another facility.
Lipidomics was at 50um resolution on a Bruker TOF I'm not familiar with, but they did both MS1 and MS/MS analysis. The deep visual proteomics was done on approximately 500 cell cuts. Pretty cool since we learned yesterday that brain cells are small. That's 25nanogram or so? Small!
TMTPro was used for the proteomics with MS2 on an Orbitrap Fusion II (Lumos) system and found around 300 differential proteins of interest that the authors seem interested in. >40k peptides are reported, which is pretty darned good from sections this small on this hardware. If you're thinking of taking the spatial proteomics plunge this seems like a great resource for taking that step.
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