I had a short day last Friday because one of the kids that plays Paw Patrol with my kid had a positive Covid test. Wait. Is that still a thing? Yes it is. So the very first thing I wondered was "I wonder what the virus does to hamsters???"
Proteomics to the rescue!
If you're not good at reading or a conservative or both, the two seem to correspond, I will clarify that I'm being facetious here.
I legitimately think that some friends and I were among the first people in the US to have Covid. There was an international trip and one person had just flown in a very long way east from a meeting and we were so so so sick. But there weren't tests then, and who knows? And who knows what other people fall in that category of "possibly had the virus, but we'll never actually know?" How do you do "changed by virus" studies on people who may have had it? Or may not?
Apparently these poor little rodents express all the correct proteins to make them a good model of something that can get the virus, but probably hasn't. So the animal testing is justified by the fact it's probably impossible to build a good human cohort that hasn't been exposed. I hate animals models. Abhor. Loathe. But I also can't CRISPR the most abundant protein in a human's hippocampus either.
Animal model...is...I'll grudgingly admit, the only way to do some things.
Proteomics was done by DIA on an Orbitrap III (Eclipse), pooled samples were used to make a spectral library then DIA was used for quan. Phosphoproteomics was also performed and a pile of IHC and validation was also performed. Solid looking study. Easy to write a silly headline. A+.
No comments:
Post a Comment