One issue with phosphoproteomics enrichment methods is the large required sample input. There are other issues, such as the mod is intrinsically transient by evolutionary design, but this group totally tackled the first one.
I figured that anything sounding this complicated would require me to wait until a commercial kit was available, but the set up sounds surprisingly (shockingly?) simple. The paper is open access, so you can get that part list yourself if you are interested.
Another reason to check out this paper is that they coat their low-bind plastic in n-Dodecyl-Beta-Maltoside (DDM) and show that helps a lot. If you're interested in just reducing your surface loss binding stuff but possibly also worried about the longitudinal effects of a recently described detergent blasting into your mass spectrometer, here is a process to coat and discard the active solution!
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