This has come up recently. Ion traps are SUPER SENSITIVE. Why wouldn't we use those for single cell applications? Because the only way to get one is to buy one attached to the back of an Orbitrap? Valid point, but it didn't used to be that way! Proof? There is a stand-alone linear ion trap on Ebay for under $10k!
Now, what would cost more? Buying a $1.6M Orbitrap with an ion trap stuck on the back of it, or buying a couple of ion traps and finding someone who could help you make it do DIA? One should be less expensive than the other, but I digress, sort of, BECAUSE here is yet more evidence that ion traps are great for single cell proteomics!
Seriously, it works surprisingly well. Now, there is probably some in-between here as well, because you could probably use any hybrid that has an ion trap in it and get similar results.
*HINT* Which ones do you think work better for this? The ones where the ion beam goes right into the ion trap? Or the one where the ion trap is 3 feet away from the origination of the ion beam? I think it's the one that is tougher to make do DIA, unfortunately.
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