Sporulating bacteria are responsible for all sorts of diseases. The little spores are dehydrated, surrounded by ridiculous numbers of layers of bacterial cell wall and other protective mechanisms that can make them resistant to extreme conditions. Some bacterial spores have been shown to resume vegetative growth after remaining dormant for thousands of years. Despite this, there is a lot we don't know about them.
Time to break out some new toys and do proteomics on them again, right?
In this case, this group used a TIMSTOF Pro and 55 minute gradient, which probably seems like overkill for a bacillus that only has 3,000 genes, but hey, it's just an hour per run!
What did they get for this? Well, this paper is extremely short and just kind of shows that proteomics can support 50 years of molecular biology used to figure out this model organism (B. subtilis), but all the files are on ProteomeXchange here!