Hey! Remember TOMAHAQ?!? It's the technique that Gygi lab developed for using internally triggered targeting for quan, kinda like IS-PRM, but then the thing you target is actually TMT labeled?!?
You can download the original TOMAHAQ paper from Molecular Cell here.
Okay -- so TOMAHAQ is suuuper smart. Unfortunately -- outside of Gygi lab, Rosa Viner, and Simion Kreimer -- I'm not sure anyone else ever used it. Why?
1) The experiment was nearly impossible to set up.
2) The data was nearly impossible to process.
What's on my desktop? Right now? 400 icons and a picture of Isaiah TomCat sitting on the head of a pug...AND...
.....A WAY TO EASILY CREATE TOMAHAQ EXPERIMENTS AND PROCESS THE DATA! Which is the reason I'm writing this!
Multiplexed internal standard triggered multiplexed targeted proteomics. For everyone. You can get the TOMAHAQ Companion software here. If you're paywalled for the paper, there is enough information within the GitHub download to set everything up.
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