Monday, May 12, 2014

What factors does Percolator consider when used with SequestHT?


I received this cool list today from a colleague.  This is the list of features from SequestHT that Percolator uses in its rescoring algorithm.

Delta Cn2
 Binomial score
 % Isolation Interference
 Observed Mass
 Delta Mass [Da]
 Delta Mass [ppm]
 Absolute Delta Mass [Da]
 Absolute Delta Mass [ppm]
 Peptide Length
 Is z=1
 Is z=2
 Is z=3
 Is z=4
 Is z=5
 Is z>5
 # Missed Cleavages
 Log Peptides Matched
 Log TIC
 Fraction Matched Intensity
 Fragment Coverage Series ABC
 Fragment Coverage Series XYZ
 Log Matched Fragment Series Intensities ABC
 Log Matched Fragment Series Intensities XYZ
 Longest Sequence Series ABC
 Longest Sequence Series XYZ
 IQR Fragment Delta Mass [Da]
 IQR Fragment Delta Mass [ppm]
 Mean Fragment Delta Mass [Da]
 Mean Fragment Delta Mass [ppm]
 Mean Absolute Fragment Delta Mass [Da]
 Mean Absolute Fragment Delta Mass [ppm]

 XCorr

Thanks, Kai, for generating this list!

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