Tuesday, May 27, 2014
IMAC or TiO2 enrichment for phosphopeptides? How 'bout both in one step?
Heck, this lab is good! Albert Heck, that is!
While all of us were sitting around trying to decide whether to do IMAC phosphopeptide enrichment first or TiO2 enrichment, or even which one we would use if we didn't have time to do both, the Heck lab was making beads that use both.
In this new paper, currently in press at MCP (and open access now) the Heck lab shows that these new reagents work really really well. They demonstrate the monitoring of >10k phosphosites over 6 different points in a time course experiment.
Totally worth checking out.