You've probably seen talks that this was coming. Here it is! Wait. What month is it? I've been busy. Not the newest news, but still worth talking about!
Don't get distracted by the Astral - that's not what I want to talk about here. What I want to talk about is how if you prime an EvoTip and then leave it sitting in warm water you can just put your lysis buffer and trypsin in the tip and load your cells. A mere hour later -- you can complete your EvoTip peptide loading and wash steps and run your tip!
The numbers are awesome. Part of that is the Astral we aren't talking about, obviously. But if you're getting 8,000 proteins with a direct injection on anything your digestion worked pretty darned well.
Hell yes we're trying it right now.
Now, if you want to do proteomics with me I pretty much require that you use S-Traps. The darned things work every time and people who have never done a prep in their life can buy the complete kit from ProtiFi and it almost always works. I LOVE that consistency, but a commercial S-Trap in bulk will set you back about $3/sample and one of those complete kits are closer to $20/sample. You throw in $7/sample for trypsin and then your $2 for each EvoTip and your sample prep isn't cheap.
If you can load the EvoTip alone, you'll obviously only get one shot per tip, so replicates will pile up to make the S-Trap a much better bargain, but there are places where doing everything on one tip will be a huge cost and time savings!
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