Wednesday, October 26, 2022

Linear ion trap data independent acquisition for low level samples!


Crap. I thought I had this niche locked down where I apply alternative mass analyzers with superior characteristics to the few things Orbitraps aren't the best at. 

This group fired up a linear ion trap and did some low load DIA analysis. Ion traps are always going ot be more sensitive than Orbitraps. The ions strike the detector in the former, and pass by the detector in the latter

But DIA? On an ion trap? Sure! Why not? 

My first concern would be specificity, but that's always my concern with DIA anyway. You lose some mass accuracy, but if you're going faster can you make up for that with smaller windows? 

The nitpicky crowd of grumpy people who review for JPR would probably have really looked into inflation of FDR, etc., and not let a lot of bad data by. You aren't supposed to think those things, but there are some journals where you find out that you forgot to unlock one of your repositories during the peer review process, and some you only find out years later when some weirdo actually wants the files.  

The FAIMS Pro source they employed also totally has to help. 

I'm really would like to spend more time on this, but I'm having the sort of week where I have fondly remembered washing dishes in the back of an un-airconditioned restaurant in the summers for $5.25 an hour. 

Super cool work, and what a great idea for that linear ion trap you have sitting in your garage! 

1 comment:

  1. There is also this super nice work by Borràs ( and his lecture on the subject (YT, MaxQuant summer school). In our lab we have a LTQ Orbitrap Elite, I was just thinking of trying and finding out what would come out of it.