Have you ever read a study and been both impressed by the ingenuity and quality of the work while simultaneously hating every well-written word and beautiful picture in it?
I love WinO because it looks like a well-executed and extremely clever study. I also hate it because [
excessive angry profanitities dedacted after I realized when I type profanities on this admittedly loud keyboard it scares the raccoon family that has set up shop in my attic. Turns out I haven't been hallucinating stomping in my attic, I just saw a tiny clumsy raccoon! SUPER cute, but I imagine having them up there is subideal for at least a couple reasons....] I thought that maybe, just maybe, at 17,832 variations of how to prep mammalian cells for LCMS based proteomics we'd finally gotten them all and we surely didn't need another way to introduce new inter-/intra-lab variability into our experiments, but here's another one!
Am I going to be a vulgar hypocrite and try this method? Fuck yes I am. I ordered oil yesterday to set up some collaborator's samples right now. This is the best way of doing things, because I obviously didn't prep their cells like this last time, I didn't even know it was possible, so I don't even give any of you the chance to undermine me and my results, I'll do it myself.
After I try to get a picture of a baby raccoon. Dude was trying to get water out of our frozen pond, I think, so I'll need to put out a heated water bowl or something.