Sunday, November 10, 2019

Precise protein turnover -- IN LIVE ANIMALS -- the ultimate protocol!


Do you have 4-5 weeks?

Do you need to get an absolute understanding of the rates of protein turn-over IN A LIVING ANIMAL SYSTEM?

This isn't the first technique for protein turnover measurements. This may be, however, the most complete picture that we've been able to get.


If your strengths aren't exactly centered in the wet lab aspect of proteomics does this look a little bit like a nightmare? Yes. I can confirm. However, it's only the first 70 steps in the protocol that will negatively affect my already erratic sleep patterns -- at 71 we get to the data processing....yeah...it's MATLAB...but it's already all done for you!

What do you get out of this? A comprehensive and precise measurement of protein turnover in an entire organism -- like, for real, whatever organ or system you care about -- up to and including ALL of them.

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