Wednesday, August 15, 2018

NextGen NanoHPLC is here -- its EvoSeP time!

Once upon a time, HPLC companies focused on making really good HPLCs. They didn't waste everyone's time making terrible mass spectrometers.

Likewise, mass spectrometry companies kind of stuck to what they were good at, maybe they'd make an HPLC, but we'd all laugh at it, buy a real one from an HPLC company and everything was just fine.

At some point these businesses all got really greedy and tried to do everything and they all failed. Now you've got a choice, buy a great HPLC system with a turd of a mass spec attached to it, or buy the best mass spec in the world and get the unsupported virtually nonfunctional atrocity that the Walmart of Science has turned the once-great EasyNLC into. And you really don't have another option, since the vendors -- now in direct competition somehow -- have no incentive to develop compatibility. No other option --

Until now.

EvoSep!!  And this paper shows that some of our European friends are looking at this awesome little company as a way to ditch the HPLC monopolies and get crazy fast comprehensive proteomes in rocket fast time.

EvoSep is it's own company, is working to be compatible with everyone and has the following advantages over everyone:

1) FAST LOADING (what do you mean, 40 minutes to load a sample is a problem?!?)
2) No carryover (what? how is that possible in 2018?)
3) Some new technological advances!  (shirking the last decade's pattern of HPLC advances -- namely lowering the performance by subbing in cheaper components and focusing on profits over customer support staff --  but -- oh my gosh -- they CHANGED THE COLOR OF THE BOX! And instituted telemarketing?!? Science!)

I've kind of rampaged about how amazing the EvoSeP is here before. And that was before all these awesome people DID PROTEOMICS WITH IT.

If you read the HF-X paper out of Jesper Olsen's lab there is this really sad/funny part in it -- they can't shorten the gradients any more -- because the poor SleasyNano requires 22 minutes to wash it's needle (or something, I haven't read it in a while), so you might as well run a 22 minute gradient since you can't inject another sample anyway.

What if your autosampler was faster than your separation gradient? What if you could PRE-FORM your gradients so they're ready to go? What if your next sample was pulled up and waiting for the instant the last run was about to end?

Well -- then you could actually make use of the HF-X's 42.4 Hz acquisition speed. Maybe you could knock out a complete proteome (>10,000 proteins) in a few hours.

OR in the 20 minutes it takes your current system TO WASH IT'S NEEDLE you could ID 5,000 proteins. For real. 20 minutes.

Time to take my NanoLCs out in a field....

1 comment:

  1. 92 minutes of applause to you, Ben! This post is just awesome!
    And congratulations to the Evosep team; they developed a really intelligent instrument.