Sunday, October 11, 2026

narrow window DIA phosphoproteomics of over 30 cancer cell lines!


Do you need a phosphopeptide spectral library? Holy shit, this group decided to just do phosphopeptide enrichment on everything with 2.5 Da narrow window DIA.

They even went slow on the Astral (30SPD, so 44 minutes?) I don't have time to check how easy it is to parse the data out. Like, am I looking for every phosphopeptide that can appear in every cancer (or some IPSC) cell line? Or do I want to search my liver stuff against the liver library? I legitimately don't know the answer to that from either the biological side or from the ion/false discovery statistics side. Either way, this looks like a fantastic new resource. 



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