I pitched mass spec based spatial proteomics a while back at a building where they have a whole ton of microscopes that are cooled with cryogens. Supposedly someone in the audience invented the whole idea of making a microscope super cold. A Wikipedia suggests that isn't all that unlikely. Right zipcode for sure. When they started asking questions about spatial resolution and I enthusiastically gave the best I'd ever seen, it sucked all the interest out of the entire room.
For real, I think they considered not validating my parking. The microscopy people can't look at a lot of proteins at once, but when they look at them they want to be orders of magnitude below the best laser pulse our mass specs get.
What if you want to look at more than one protein at a time in microscopy?
You get SUM-PAINT, I guess? They get some stupidly high spatial resolution while using oligonucleotide barcodes behind to label your targets. Ridiculously beautiful pictures and - I don't know if this is a great paper or approach, but it give some perspective on scale. There are a lot of 5nm pixels (their scale) inside of a 20 micrometer one....
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