This isn't super new, but a colleague sent it to me and it's really really cool.
The liver is really weird and even though from a microscopic level it looks like a bag of square (they like "cuboidal") cells all stacked like bricks row after row forever, these cells are very different depending on where they are. These big bricks of cells are also packed full of mitochondria and may have hundreds of them per cell. This group used spatial sorting to get piles of hepatocytes from different zones THEN did mitochondrial enrichment THEN did (TMT) proteomics and phosphoproteomics.
There are big differences in mitochondria depending on where the cells are spatially in the liver. I was going through the methods and thought something like "wow! someone knew what they were doing! why don't I recognize any of these names?" I re-read the names. I know the 9th author. Wait. How is a mostly proteomics paper...PI...is....9th...author....meh...probably politics stuff..... There is pretty ....confocal...microscopy pictures, though, and those can be hard to do as well, you have to sit in the dark forever and take pictures and some people were deeply offended if you listen to music while you do so! I have no idea what the top panels mean, but I do like pastels (see top panel). For real, really nice work though and something we'll definitely discuss in a lab meeting in the spring semester, for multiple reasons!
No comments:
Post a Comment