Antibody and antibody drug conjugate (ADC) drugs are EVERYWHERE. You can't watch 3 minutes of YouTube or Television (is that still a thing? I can't believe YouTube still exists at all either, how hard would it be to replace it with something that was good?)
Do you have to run 1 hour or 2 hour gradients of digests to work them out? If so, you sure couldn't keep up with a big multi-lot multi-batch generation facility.
Could you just digest and direct infuse? Probably! But how would you analyze those data? With iFishMass!
They start off by doing some antibodies and ADCs with a standard nanoLC setup and then they move over to the NanoMate. Remember those? They are typically used for intact protein analysis.
Turns out that on a Tribrid it works pretty great for simpler single protein digests. Some great news about the software for me is that you convert the data to a universal format before you put it into the software (one of the formats that starts with an X and ends in an ML. I forget which one.
And iFishMass is up and available to run here. Funny point in the manuscript is where it says something like "the easiest way to install it is..." which is super handy and I'm glad the reviewers let them sneak in a helpful tip there.
No comments:
Post a Comment