If you've seen me talk about single cell proteomics, I like to point out the proteins I can see (50,000 copies and up) and I make a joke suggesting you come back in a decade if you're interested in transcription factors.
They turn on transcription at your DNA...you only have a couple copies of each gene. In many, most cases, we only expect a few copies of each of those proteins in each cell - certainly not tens of thousands!
Yes this preprint demonstrates measuring them in global single cell data!
How? Well...it's complicated. But basically it looks like we've got MS1 - DIA - and then what appears to be heavy isotope triggered wide window PRMs all in one cycle. I *think* the instrument sees the heavy spiked peptides and then moved a PRM window over to where the light should be.
For you hardware nerds you may be interested in the fact this preprint seems to feature an Orbitrap Eclipse, and Orbitrap Asstral and something called an Orbitrap Astral Zoom, which might suggest we'll see an Asstral 2 at ASMS tomorrow? I guess we'll see!
The first author will be presenting it as poster WP724 (Wednesday? Poster 724? I think!) if you're in Baltimore!
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