Did some weird old doctor just tell you they have a crapload of formalin fixed paraffin embedded tissues they'd like to do proteomics on? Or worse....phosphoproteomics? (Eek)
What modern shotgun proteomics prep method is the best today?
Don't test them all for yourself! That would take forever! Reading this would be faster.
Great and important work! The statement "All methods performed well, except for SP3 due to insufficient tissue lysis." is a bit odd though. Not surprised the protocol didn't work well if the first step before the protocol was not done right. So the other protocols work good with insufficient tissue lysis or it was done differently? Idk.
ReplyDelete