Thursday, August 15, 2024

Amazing new (to me) videos on mass spec physics - and operations!

 I'm adding this stuff to the tutorials tabs over there somewhere --> 

Check out this stuff! 

First, the ShortChemistry channel! 


Alan Doucette does this and they are fantastic. Want a walkthrough from sector trajectory to how the fast fourier transform gets you a mass in an Orbitrap - maybe with a short clip from when the Simpson were good to make sure the point gets driven home? This is a fantastic technical chemist with a great grasp on the physics who is an even better instructor. 

And this is actually brand new - and I love it! I don't know when I last watched 3 hours of anything....


 From the title you'd think....that sounds super boring, but this might be the single best walkthrough of 

1) How to operate a TIMSTOF (with a NanoElute2) 

2) How to set up MaxQuant.Live targeting (on an Exploris 480)

3) And how to run the QC scripts (in R...🤮🤮🤮...but for the second time this week, a blog post will say...and it might be worth it.... ) from NorthEastern/PSTI to look at multiplexed single cell data.

Given blog metrics, very very few people care about number 3, but Arturo is obviously an expert level TIMSTOF operator - and here is an hour of his tips and tricks. If you've never hacked operated an instrument with MaxQuant.Live, Andrew provides a better breakdown of how to do it (on an Exploris 480!) than either the original authors or my pitiful slide decks. My only criticism is Andrew's posture when he's sitting. First hand I know there is a price for that stuff and you're lucky if you don't have to pay up for a couple of decades. Otherwise, it's a fantastic walkthrough that should make you jealous you weren't in Boston in June to see it in person. 

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