Tuesday, March 10, 2020
Use the SCoPE-MS concept to BOOST! Tyrosine Phosphoproteomics!!
They got it to work for Tyrosine phosphoproteomics!! I've worked on this for S/T and it's been -- meh -- and I know another scientist (who actually tipped me off to this idea) who has had some results but it hasn't been a clear winner -- but where we really really need more signal?
Tyrosine phosphoproteomics sucks! The signal is low and they fragment poorly. We can't fix all of this with more signal, but it definitely won't hurt.
Check this out!
As with any of study and, in particular, with anything employing differing levels of loading -- you have to painstakingly optimize everything to get it to work right. And that's what you'll find here!