Tuesday, March 24, 2020

COVID-19 (SARS-CoV-2) Protein Interaction and Drug Repurposing study!

It is truly freaking amazing how fast some of these studies for COVID-19 / SARS-CoV-2 /2019-nCOV, are coming together.

As I'm waiting patiently for even the RAW files to download, you have to think -- how the heck did they get this study done this fast!?!??

Okay -- so 75 people working on it? That could be helpful!

Each protein from the virus was cloned and used as a bait -- whoa...they even cloned the predicted cleavage sites separately....and they tested all these drugs....?...wow.....

A KingFisher was used to help automate the pull-downs/IPs/AEs, whatever you like to call them.

The digestions were performed on-bead and 2 LCMS systems were used. One is a QE Plus system running 75 minute gradients. I'm a little unclear on if 2 identical systems were used or if the second system was something different.

Downstream analysis used SAINT and MIST and MaxQuant and protein complexes were cross-referenced with CORUM. And the bioinformaticians on this weren't sitting around. The number of programs used in the downstream is staggering....

What you want is the ProteomeXchange data and it's all here.

Aren't a proteomics wizard? Ummm..you might have come to the wrong weird blog. But if you just want the Protein Protein interaction data -- they were uploaded to NDex!

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