Okay -- I finally did it. A little more than 2 years later, I finally got to a point where I felt guilty about my rambling about the relatively low quality of the first TIMSTOF data that I had access to and took the original post down.
I'll save it for historic purposes, but Bruker has improved or just fixed every problem that I had with the original instrument. The mass accuracy is better, the speed is even faster and there are options for data processing
Also, the post was just kind of crappy. This placeholder is worse.
Hi Ben,
ReplyDeleteyou are able to adjust the TIMS scan time which determines the number TOF scans within a TIMS duty cycle. Results have shown that 50ms are much more benefical for number of unique precursors compared to higher TIMS duty cycle time. You might want to check this out https://www.biorxiv.org/content/early/2018/06/01/336743
Regarding the monoisotopic isolation - Since you have the ion mobility dimension they is a fewer need to isolate monoisotopically that is the major advantage of the technology.
Cheers
Ben,
ReplyDeleteDid you ever get a chance to run the PASEF data?
Best,
~Mick