Somebody went all out on diaPASEF peptidomics exploration - analyzing baby formula!

 

I was initially a little appalled by the amount of optimization of diaPASEF windows in this paper, but I read more of it while walking a mean little dog in the rain and there is a lot here. 

Food science is very different that your run-of-the-mill proteomics samples. Often the thing you're worried about is the allergenic peptidome, and those sure don't look like tryptic peptides. It's likely that this team ran some baby formula using the default methods on the instrument and they didn't see a single thing of interest.

They had to go back to the drawing board do wide IMS window DDA and build their windows for their targets iteratively until they had a good method.

Even in this context, I have to admit there are just too many purple DIA windows to make this more than a useful reference for the next time we have to look at something singly charged and 10-50 amino acids long. I certainly don't want to read the whole thing until that happens, but I'm glad to know it's out there!