...well...I didn't know that you could do this....
And - wow - has ChemRXIV gotten a glow up! It used to be the ugliest duckling of the preprinting world, but now it's totally modern and legible!
For this study something called a Q Exactive HUMR with charge detection was used to work out these assembled protein complexes. Flipping everything I know about Orbitrap intact protein work on it's head, this group ran the charge detection thingy at 200,000 resolution with direct nanoinfusion for possibly as long as 30 minutes.
I've done a lot of intact mAB work, somehow almost always out of the goodness of my heart. Actually - advice to younger scientists who might be way too nice and willing to help people - If anyone wants you to get a good intact mass of an mAB - especially if they are a startup - payment up front every single time. And if they said they worked with me, definitely reach out so I can tell you about how I took work from a nice CRO or three and got $0.00 for it and I'll suggest you charge them 100x what you're thinking.
Wait. What was I typing about? OH YEAH!
Okay, so on mABs on a regular Orbitrap I generally run 15,000 resolution, when KRAS will look great at 70,000. Intact mABs are 10x larger than KRAS and those spare molecules in the Orbitrap wreak havoc on something that large. On an Exactive EMR, the biggest thing I ever did was maybe 250kDa and I had to run it at 7,000 resolution or something to get it to resolve
So whatever this charge thingy flips that upside down! 200,000 resolution! That's crazy! And they step through collision energies to get data on this heavily glycosylated complex! Super cool work.
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