I had a super early version of the 7600. I had it before it could do ZenoPulsing/ ZenoTrapping in DIA. It was a nice instrument and super ridiculoulsy great at targeted proteomics. That PRM thing (mRmHR?) loved Skyline and it processed data super fast. But it wasn't up there with my TIMSTOFs. Not really. You could doulble the gradient length and increase the amount you loaded on column by 2-4x and get the same coverage as the TIMSTOF Flex with the XR catrdidge.
You sure as hell couldn't get 4,700 protein groups at 500 SPD from 200ng of K562! Make no mistake thsi is far higher throughput than the "next gen" proteomics solutions out there. This is more than 10x more samples/day than Illumina's new protein prep - AND this thing can QUANTIFY proteins. Friendly reminder that Illumina Protein Prep only detects proteins. It does NOT QUANTIFY proteins in any meaningful way. O-Link can provide meaningful quantitative responses, but on a single instrument setup 500 SPD is going to be 2x - 4x the throughput (though I've heard this is improving with 1536 well plate preps or something).
Something weird on the 7600 was that it had a lot of the optics from the earlier generation QQQs. The 8600 doesn't. It's got the new stuff and on top of the speed they're showing off, this thing can hit solid coverage at single cell equivalents. They even prep single HeLa cells in 1 cell, 3 cell, 10 cell and stuff and it looks good. Also there is some 3 proteome digest stuff that looks pretty solid - obviously better quantitative accuracy at the lower speeds. CVs look decent. If you could nitpick these results you could say that the ion mobility equipped instruments do get cleaner spectra and tighter CVs but - for real - SCIEX seems to have a legitimate competitor for the other company flagships. I was about to make a bunch of highlander jokes following some recent software company acquisitions but it looks like there is still legitimate competition out there in the world!
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