If you've ever been to this blog before, you might have encountered the fact that I despite nanoflow liquid chromatography.
I rarely quote the text of a paper completely, but I'm going to do it with this great new one.
Begin quote:
When I started my first proteomics experiment in 2007 or something, I thought "man...these motherfuckers are dumb....this is the shittiest 'separation' I've ever heard of". But you had to do it...probably...because the mass spectrometers needed 4 kilograms of protein separated over 6 weeks of chromatography time to detect 3 proteins besides albumin.
Now that mass spectrometers don't completely suck, maybe, just maybe, we should go back and stop doing shitty unsustainable chromatography that only has the benefit of extremely poor reproducibility?
End quote.
Even though nobody asked, but because it's related to this topic, I highly suggest this tutorial https://doi.org/10.1021/acs.jproteome.2c00407 and this paper https://doi.org/10.1021/acs.analchem.8b00525 both by Lenčo et al. To whom I am not affiliated but maybe we can all start walking towards a nano-flow-less world
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