Rapid microglia phagosome proteomics!

 

A big thank you to Aleksandra Nita-Lazar for suggesting this as one of the most impactful papers of 2024 that didn't make my initial list! 

Again, the less I type here the better on the physiology/interpretation point, but from the introduction it's pretty clear that: 1)  microglia phagosomes are super important biologically and in a "plethora of brain pathologies" (stolen from the abstract) and 2) you'd think we'd know a lot about them, and we do not.

The part I do understand here is that a LOT of techniques were employed including proteomics on the purified phagosomes. That part was performed using a QE HF-X using around 100 minute total gradients. I like the fact that a very large inline traps were used (6cm x 100um) and I think that extra pile of chromatographic resolution in the trap helps make a 14cm x 75um separation column better match the relatively long gradients employed. DDA was used and downstream analysis was performed with MaxQuant and Perseus

LCMS metabolomics was also performed on a QE Classic exclusively using HILIC separation (I think) with data analysis through Compound Discoverer. There is a typo in the MS1 resolution, but otherwise it's all really well detailed. 

I love me some multi-omics data integration and there is a lot here to integrate. 

Aleksandra said that there are fantastic biological findings here and the authors seem equally enamored with this whole pile of data we have on these important subcellular compartments.