SpectroNaut 19 has super smart new PTM functionality!

 

Happy New Year, y'all! Wow, I'm busy with this move, it's 1/7/25?? Typing fast! 

Okay, so a while back I did a funny thing where I put up a survey on LinkedIn and also on some other social media platform and got two very different replies from the respective communities. 

My exaggerated summary is: 

Industry people seem to ALWAYS normalize the amount of PTM (for example, phospho) versus the total amount of protein present.

Responses from academics were more like - 

If you're in that latter category, here is the thing from my perspective - you never ever see a western blot for the MAPK pathways where someone just blots against phosphoSTAT, phosphoERK, you always see this sort of thing (taken from this paper) 

They do this because, presumably, having 10x more ERK protein at 1% phosphorylation site occupancy and no change in ERK protein but a 10% increase in phospho-ERK means something important. I honestly don't know, I just always do this because the western blot people do. I do it with an Excel sheet I made like 10 years ago and a Shiny App a previous student and I made more recently. 

SpectroNaut now does really really smart things (smarter than I do) automatically! There is more information about this here!