Holy shit. I'm way too busy today to spend the time on it that it deserves, but I'll set some files to download until I do...
Up front - I'm biased. IMP is one of my favorite places in the world and this study features both friends and a personal hero or two, but this is a sick paper.
I do very much appreciate how clear the authors are here about cell size, etc., they don't average 5,300 proteins per A549 cell, but they do get that on the largest ones with the highest relative protein content.
There is a lot to unpack here. They start with peptide dilutions, but then move to multi- proteome mixture to make sure that what they're seeing at low loads are relevant quantitatively. All throughout they are transparent about carryover and other factors that mean they are seeing proteins in their "blanks".
Y'all, it wasn't that long ago when I wasn't getting 5,300 protein groups single shot proteomics on A549 cell lysates regardless of how many cells I started with. The fact a big A549 cell could give you anywhere near those numbers is just absurd.
No comments:
Post a Comment