I totally dig this new study in...dogrammit....it's Elfseverer again....I swear, I'm not doing this to discriminate against people in the EU. Papers show up in my feed and I try to read them without looking at the author names or what journal it came from to avoid my powerful subconscious biases -- and people still submit cool things to these journals. Wait -- shit -- I may have just been emailed that I was author 84/219 on something that went to one...I should write less and read more emails....
What was I....THIS!
This team takes a look at running the same sample, gradient, instrument parameters and data processing scheme and just switches up buffer B.
Quick note: If you've got a
However, this brave team obviously checked with their LC manufacturer, found it was all good and swapped up the buffers on their LC and -- seriously 30% increase in HeLa IDs in what was a sub-2 hour gradient by swapping acetonitile with methanol and then acetone.
What surprises me the most, perhaps, is that the elution profile is even similar. If at 3% acetone, all the peptides came off in one single peak, I'd be less surprised. The detector was an Orbitrap Velos running in high/low mode and the RAW files are available on ProteomeXchange here (but have yet to go live as of the time of this post's rambling).