Saturday, June 15, 2019
BASIL -- Use TMT to amplify phosphopeptide IDs!
YES. BASIL is not only tasty it's also a great way to improve your phosphoproteomics! You should check out the paper here.
Okay -- so you know how SCOPE-MS works for other people (not me, but it's definitely my sample prep inabilities)? In ScoPE you load a lot of signal in one channel of TMT. Since all your peptides get combined in the MS/MS, the high channel provides enough signal for identification and then the reporter ions allow you to quantify things that would be below your S/N for an ID.
What if you did the same thing for phosphoproteomics? That's BASIL.