Thursday, December 28, 2017

Microwave digestion returns -- and appears really useful for single protein mapping!

I was initially skeptical when a friend told me I should check out this paper.

I've been down the microwave digestion route before. And, while I may have primarily been young(er)  and stupid(er), I still believe that the microwave cooked off my phosphorylations.

However -- this new study is pretty convincing that the technique has real value! 

Unlike enzymatic digestion which will occur reliably at specific residues, put some formic acid in with your proteins and microwave them -- and the proteins break all over the place. Reactive oxygen/ I don't know, but it does really work. (but it might cook off the phosphos first )

No comments:

Post a Comment