Sunday, March 9, 2014
Comparative proteomic analysis reveals characteristic molecular changes accompanying the transformation of nonmalignant to cancer lung cells
Interesting paper from Li Zhang's lab. Straight up comparison between two cell lines from the same patient -- a normal cell line and a lung cancer cell line.
iTRAQ labeling and Q-TOF for analysis isn't the most sensitive way of doing things but the bioinformatics afterward makes up for it. They get quan on only high abundance proteins, but the quantitative differences let them point up stream at some really well known (and less well-known) cancer checkpoint proteins.
A great paper that shows how far you can go with some extra elbow grease on older equipment. Direct link to the paper here.
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